The Portugal international, 27, was absent from Tuesday's open training session after picking up a minor issue. Manager Arne Slot expects Jota to be in the squad to face the Serie A club.
"Diogo missed two sessions after the Wolves game as he took a knock," Slot said. "I'm expecting him to be with us [for the game]." Liverpool forward Federico Chiesa also missed Tuesday's training session and is ruled out of the Bologna match.
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"Federico trained with us and came in today with some problems and I'm not expecting him to be in the team," added Slot.
"It is a pity for us and for him as well as he was looking forward to a Champions League night at Anfield, especially against an Italian team.
"I'm not expecting him to be out for a very long period of time, but this game is not possible for him and we have to wait to see if he can be in the team on Saturday [against Crystal Palace]."
Liverpool are looking to make it back-to-back wins in the Champions League after beating AC Milan in their opening fixture.
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